Extracellular vesicles (EVs) are membrane-derived particles surrounded by a phospholipid bilayer that are released by cells. We use DiO dye to efficiently label EV membranes. Then the excess unbound dyes are fast removed through our excellent Spin Columns from labeled exosome preparations, which separate molecules on the basis of the differences in size. Contaminant removal is easier and faster than traditional clean-up methods such as ultracentrifugation, spin filters.

With a highly specific membrane labeling and very low background levels, the kit can be used for most applications that require visualization of labeled EVs for tracking studies

The carbocyanine dye DiO poses highly lipophilic nature and is widely used to stain cell membranes. The dye uniformly labels cells via lateral diffusion in the plasma membrane. The fluorescence intensity of free DiO is very weak, but greatly enhanced when incorporated into membranes. DiO emits green fluorescence when excited, its maximum excitation and emission wavelength is 484nm and 501nm, respectively.